Abstract: | The bioaccumulation and excretion of antimony by the freshwater alga Chlorella vulgaris , which had been isolated from an arsenic-polluted environment, are described. When this alga was cultured in a medium containing 50 μg cm−3 of antimony(III) for 14 days, it was found that Chlorella vulgaris bioaccumulated antimony at concentrations up to 12 000 μg Sb g−1 dry wt after six days' incubation. The antimony concentration in Chlorella vulgaris decreased from 2570 to 1610 μg Sb g−1 dry wt after the cells were transferred to an antimony-free medium. We found that the excreted antimony consists of 40% antimony(V) and 60% antimony(III). This means that the highly toxic antimony(III) was converted to the less toxic antimony (V) by the living organism. Antimony accumulated in living Chlorella vulgaris cells was solvent-fractionated with chloroform/methanol (2:1), and the extract residue was fractionated with 1% sodium dodecyl sulfate (SDS). Gel-filtration chromatography of the solubilized part showed that antimony was combined with proteins whose molecular weight was around 4×104 in the antimony-accumulated living cells. © 1997 by John Wiley & Sons, Ltd. |