TEMPERATURE DEPENDENCE OF CHLOROPHYLL FLUORESCENCE FROM THE LIGHT HARVESTING COMPLEX II OF HIGHER PLANTS |
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| Authors: | Alexander V Ruban Jan P Dekker Peter Horton Rienk VAN Grondelle |
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| Institution: | Department of Physics and Astronomy, Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081 HV Amsterdam, The Netherlands;Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, PO Box 594, Western Bank, Sheffield, S10 2UH, UK |
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| Abstract: | Abstract— Chlorophyll fluorescence spectra of LCHII, the light harvesting complex of photosystem II, have been recorded in the aggregated and trimeric forms for a range of temperatures from 293 to 4 K. At least five long-wavelength emitters in the 682–702 nm region with different temperature dependencies were found in the spectra of the aggregates. At 293 K the yield of LCHII trimers was higher than aggregates by a factor of 4, but, upon lowering the temperature, a fluorescence rise was observed which was much stronger for LCHII aggregates than for LCHII trimers, so that at 4 K their yields were comparable. The implications of these data in terms of the function of LCHII are discussed. |
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