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Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase |
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| Authors: | Jason J Reddick Cynthia Kinsland Robb Nicewonger Todd Christian Diana M Downs Malcolm E Winkler Tadhg P Begley |
| Affiliation: | a Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, 14853, USA. b Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA. c Department of Microbiology and Molecular Genetics, University of Texas - Houston Medical School, Houston, Texas 77030-1501, USA. |
| Abstract: | The overexpression, purification and characterization of 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (HMP kinase) and 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase (HMP-P kinase) are described. Surprisingly HMP-P kinase also shows HMP kinase activity. These enzymes are useful reagents for the preparation of intermediates on the thiamin biosynthetic pathway. |
| Keywords: | Enzymes phosphorylation biosynthesis |
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