利用拉曼光谱技术检测中蒙药中朱砂的研究

朱砂有凉性、解热、收敛作用，一般以粉末状入药于中药或蒙药中。然而朱砂具有一定的毒性，因此有必要对药品中朱砂的含量进行检测分析。利用便携式拉曼光谱仪对朱砂进行了鉴别分析，获得了朱砂较为全面的分子结构振动信息。实验结果显示朱砂的拉曼光谱在253，290和343 cm-1等处出现了较强的拉曼特征峰，文中确定以此三处特征峰作为研究对象。同时，检测了蒙药八味止血红花散、朝伦-雄胡-5、外用溃疡散以及中药口腔溃疡散的拉曼光谱。结果在含有朱砂的几种蒙药八味止血红花散、朝伦-雄胡-5和外用溃疡散均检测到了朱砂的特征峰，而在不含有朱砂的中药口腔溃疡散中未检测到拉曼特征峰。此外，对口腔溃疡散中的朱砂进行半定量分析，检测极限可达到质量分数的10%左右。拟合得到了中药口腔溃疡散的拉曼光谱特征峰强度与其中朱砂的掺杂量之间的关系，获得曲线的相关系数r=0.995 9，验证了拟合结果的准确性。该拉曼光谱检测方法具有快速、简便、准确等优点，值得在中蒙药成分鉴定方面得到广泛的应用。

Detection of Cinnabars in Mongolian Medicines Using Raman Spectroscopy

Cinnabar could soothe the nerves and the powder of cinnabar is always added in traditional Chinese medicine or mongolian medicines. The surface-enhanced Raman spectrum of cinnabar was identified using a portable Raman spectrometer and most structure vibration information was obtained. The results show that the Raman peaks of cinnabars were located at 253, 290, 343 cm-1 and this three Raman characteristic peaks were selected for cinnabar identification. Meanwhile, the Raman spectra of several mongolian medicines were collected. The results showed that Raman signal of cinnabar could be observed in several mongolian medicines which contain cinnabar and Raman signal of cinnabar couldn’t be detected in several mongolian medicines without cinnabar. In addition, the cinnabar in the oral ulcer powder was semi-quantitative analyzed and the limit of detection could reach to 10% of mass fraction. The relationship between the doped amount of cinnabar in the oral ulcer powder and the Raman intensity of characteristic peak was fitted and the correlation coefficient (r) was 0.995 9, which validated the accuracy of the result. This Raman analysis method for cinnabar detection is rapid, simple and accurate and it can be applied widely in mongolian medicines determination.