A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;2. Department of Radiology, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;1. School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China;2. State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;2. Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China;3. Suzhou Institute of Biomedical Engineering and Technology (SIBET), Chinese Academy of Sciences, Suzhou, Jiangsu, 215163, China;1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China;2. School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai, 200240, PR China;3. State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, PR China;1. Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China;2. School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China;3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China |
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| Abstract: | A highly sensitive fluorescent sensor ZnDN was designed, synthesized and used for tracking intracellular zinc ions in various living cells and direct imaging of prostatic tissue in mice. ZnDN was prepared from the heterocyclic-fused naphthalimide fluorophore, and the zinc receptor, N,N-bis(2-pyridylmethyl)ethylenediamine (BPEN). Upon addition of Zn2+ to the solutions of ZnDN, a remarkable fluorescence enhancement was observed, which could be attributed to the photo-induced electron transfer (PET) mechanism. Since ZnDN exhibited high sensitivity toward Zn2+ in phosphate buffer solution, with a limit of detection of 4.0 × 10?9 mol/L, it was further applied for the imaging of exogenous and endogenous Zn2+ in different living cells. Living cells imaging experiments suggested that ZnDN could image the changes of intracellular free zinc ions, and could be used for two-photon imaging. Moreover, flow cytometry suggested that ZnDN could distinguish cancerous prostate cells from normal cells. Animal experiments indicated that ZnDN had the potential in imaging prostate tissue in vivo. |
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| Keywords: | Fluorescent probe Zinc ion Prostate cells Naphthalimide |
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