Construction of triblock copolymer-gold nanorod composites for fluorescence resonance energy transfer via pH-sensitive allosteric |
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| Affiliation: | 1. Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China;2. Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China;1. Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China;2. Department of Otolaryngology Head and Neck Surgery, China–Japan Union Hospital, Jilin University, Changchun, China;3. Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China;1. School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China;2. Guangling College, Yangzhou University, Yangzhou 225000, China;3. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China;4. Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan;1. Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China;2. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China;3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;4. Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan;5. Sichuan Selewood Technology Company Limited, Chengdu 610218, China;6. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | To explore the effects of microenvironmental adjustments on fluorescence, a pH-sensitive nanocomposite system based on fluorescence resonance energy transfer (FRET) was constructed. The model system included a modified triblock copolymer (polyhistidine-b-polyethylene glycol-b-polycaprolactone) and gold nanoparticles. A near-infrared dye was used as the donor, and spectrally matched gold nanorods, attached after C-terminus modification with α-lipoic acid, were used as the receptor to realize control of the FRET effect over the fluorescence intensity for two polymer configurational changes (i.e., “folded” and “stretched” states) in response to pH. After synthesis and characterization, we investigated the self-assembly behavior of the system. Analysis by quartz crystal microbalance revealed the pH sensitivity of the polymer, which exhibited “folding” and “stretching” states with changes in pH, providing a structural basis for the FRET effect. Fluorescence spectrophotometry investigations also revealed the regulatory impact of the assembled system on fluorescence. |
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| Keywords: | Fluorescence resonance energy transfer pH response Self-assembled nanoparticle Au nanorod Block copolymer |
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