| Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic |
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| 引用本文： | Shuangxi Wang,Bicong Zhou,Na Wang,Changmin Yu,Naidi Yang,Jian Chen,Qiong Wu,Chengwu Zhang,Lin Li,Wei Huang. Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic[J]. 中国化学快报, 2020, 31(11): 2897-2902. DOI: 10.1016/j.cclet.2020.03.037 |
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| 作者姓名： | Shuangxi Wang Bicong Zhou Na Wang Changmin Yu Naidi Yang Jian Chen Qiong Wu Chengwu Zhang Lin Li Wei Huang |
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| 作者单位： | 1. Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (Nanjing Tech);2. Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan University of Science and Technology;3. Shaanxi Institute of Flexible Electronics (SIFE), Northwestern Polytechnical University (NPU) |
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| 基金项目： | financially supported by the National Natural Science Foundation of China (Nos. 81672508 and 61601218)；;Jiangsu Provincial Foundation for Distinguished Young Scholars (No. BK20170041)；;Natural Science Basic Research Program of Shaanxi (No. 2019JM-016)；;Fundamental Research Funds for the Central Universities； |
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| 摘 要： | Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging. Herein, this study reported a novel mitochondria-targeted fluorescent probe DPACDY based on vibration-induced emission(VIE) for monitoring viscosity changes during mitochondrial autophagy. This probe contained N,N’-diphenyl-dihydrodibenzo[a,c]phenazine(DPAC) as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring. As the ambient viscosity in...
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| 收稿时间： | 2020-01-14 |
Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic |
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| Affiliation: | 1. Department of Chemistry, Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wannan Medical College, Wuhu 241002, PR China;2. Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, PR China;3. Department of Food and Environmental Engineering, Chuzhou Vocational and Technical College, Chuzhou 239000, PR China;4. Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology (IFM), Linköping University, 58183 Linköping, Sweden |
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| Abstract: | Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging. Herein, this study reported a novel mitochondria-targeted fluorescent probe DPAC-DY based on vibration-induced emission (VIE) for monitoring viscosity changes during mitochondrial autophagy. This probe contained N,N'-diphenyl- dihydrodibenzo[a,c]phenazine (DPAC) as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring. As the ambient viscosity increased, the vibration of DPAC-DY could be hindered, and subsequently resulting in the enhancement of fluorescence emission. In vitro and intracellular experiments indicated that the probe DPAC-DY showed highly sensitive response to viscosity due to VIE mechanism. Importantly, by virtue of this probe, in situ and real-time visualization of the specific viscosity dynamics during the mitochondrial autophagy process was achieved. Thus, this work provides a novel strategy for VIE-based viscosity response sensors applied to specific organelles and offers a platform for in-depth study of mitochondrial viscosity-related diseases. |
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| Keywords: | Vibration-induced emission Autophagy Viscosity Fluorescence detection |
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