An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine |
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| Affiliation: | 1. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;2. Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China;3. Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;5. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;1. Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário (SLAV/SC/LANAGRO/RS), São José, SC 88102-600, Brazil;2. Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC 88034-000, Brazil;1. CAS Center for Excellence in Nanoscience, Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, School of Pharmaceutical Sciences and the Third & Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People’s Republic of China;2. Department for Safety Supervision of Animal Products, China Animal Health and Epidemiology Center, 266032 Qingdao, People’s Republic of China;3. Department of Genetic Toxicology, Shenzhen Center for Disease Control and Prevention, 518020 Shenzhen, People’s Republic of China |
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| Abstract: | Both clenbuterol (CLB) and ractopamine (RAC) are β-adrenergic agonists. After long-term excessive intake, there will be adverse reactions such as headache, chest tightness, limb numbness, and serious life-threatening. Simultaneous detection of CLB and RAC in related samples is of great importance for human health. In this work, we outline a microfluidics-based indirect competitive immunoassay (MICI) system that can sensitively detect residual CLB and RAC in pork, swine blood and swine urine. The rapid detection of multiple samples can be achieved in one chip, which greatly improves the detection efficiency. This method has good stability and reproducibility and the microfluidic chips are easy to manufacture. The linear ranges for CLB and RAC detection by MICI are 0.1–2.5 ng/mL and 0.1–5 ng/mL, and the limits of detection (LODs) are 0.094 ng/mL and 0.091 ng/mL, respectively. This straightforward and portable immunoassay system provides a good platform for rapid detection of harmful substances in food samples. |
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| Keywords: | Microfluidic chips Simultaneous detection Indirect competitive immunoassay Clenbuterol Ractopamine |
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