| 金电极表面植物原生质体的固定化 |
| |
| 引用本文： | 张红平,祁玉兰,严曼明,韦毅,林仲华,江志裕.金电极表面植物原生质体的固定化[J].高等学校化学学报,2002,23(7):1392-1394. |
| |
| 作者姓名： | 张红平 祁玉兰 严曼明 韦毅 林仲华 江志裕 |
| |
| 作者单位： | 1. 复旦大学化学系;
2. 复旦大学生命科学院, 上海 200433;
3. 厦门大学化学系, 固体表面物理化学国家重点实验室, 厦门 361005 |
| |
| 基金项目： | 国家自然科学基金 (批准号 :2 9773 0 0 9和 2 983 3 0 60 ) |
| |
| 摘 要： | 近年来 ,基于聚电解质同携带相反电荷的基团之间的静电吸引作用组装超薄复合膜的研究受到广泛的关注 1～ 7] .但是 ,利用聚电解质来固定生物活细胞的研究尚不多见 .Wen等 8]曾在水溶液中利用聚电解质复合物构成的微囊来固定人体肿瘤活细胞 .本文将该技术应用于生物活细胞在电极表面的固定化 .利用吸附在金电极上的荷正电的聚二烯丙基二甲基氯化铵 Poly( diallyldimethylammoniumchloride) ,PDADMAC]将携带负电荷的大麦细胞固定在金电极表面 ,运用扫描隧道显微镜 ( STM)、石英晶体微天平 ( QCM)及共焦激光扫描荧光显微镜对该过程进…
|
| 关 键 词： | 聚电解质 大麦细胞 固定化 |
| 文章编号： | 0251-0790(2002)07-1392-03 |
| 收稿时间： | 2001-08-20 |
| 修稿时间： | 2001年8月20日 |
Immobilization of Plant Protoplast on Gold Electrode Surface |
| |
| ZHANG Hong Ping ,QI Yu Lan ,YAN Man Ming ,WEI Yi ,LIN Zhong Hua ,JIANG Zhi Yu.Immobilization of Plant Protoplast on Gold Electrode Surface[J].Chemical Research In Chinese Universities,2002,23(7):1392-1394. |
| |
| Authors: | ZHANG Hong Ping QI Yu Lan YAN Man Ming WEI Yi LIN Zhong Hua JIANG Zhi Yu |
| |
| Institution: | 1. Department of Chemistry;
2. Academy of Life Science, Fudan University, Shanghai 200433, China;
3. Department of Chemistry, State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen University, Xiamen 361005, ChinaLaborato |
| |
| Abstract: | Barley protoplast was immobilized on poly(dially dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMAC and the negatively charged barley protoplast. STM image reveals that PDADMAC/Au(111) surface displays a nano organized dot(4-6 nm) array structure. The processes of the PDADMAC adsorption and of the barley protoplast immobilization were monitored from the in situ QCM frequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCM were discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAC layer is 2.4 nm. |
| |
| Keywords: | Polyelectrolyte Barley protoplast Immobilization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
| 点击此处可从《高等学校化学学报》下载免费的PDF全文 |
|
