微生物远红外波段复折射率测定及模型构建

选择枯草芽孢杆菌、黑曲霉孢子和黑曲霉菌丝体等3种典型微生物制备生物样品, 采用显微红外光谱仪对3种微生物样品在2.5—15 μm波段的红外反射光谱进行测定, 基于Kramers-Kronig (K-K)关系计算得到了微生物样品在6—14 μm波段的复折射率. 以水、蛋白质和核酸的红外吸收特性为基础,根据微生物样品的 红外吸收谱线计算了微生物样品含水量. 在3种微生物远红外波段复折射率和含水量的实测数据基础上, 构建了生物颗粒在6—14 μm远红外波段的复折射率模型, 分析了模型的可靠性. 模型的构建对发展生物样品的快速分析、鉴定方法, 进而推动生物气溶胶探测、识别技术进步具有重要意义. 关键词： 光学参数 微生物复折射率模型 远红外波段

Determination and model construction of microbes' complex refractive index in far infrared band

Bacillus subtilis, Aspergillus niger spore and Aspergillus niger mycelium are chosen for samples preparation. The above three samples' reflection spectra in 2.5—15 μm band are recorded by using infrared microscopic spectrometer. From Kramers-Kronig (K-K) relationship, microbe samples' complex refractive indexes in 6—14 μm band are calculated. According to the microbe samples' absorption spectra, their water contents are calculated using the absorption characteristics of water, protein and nucleic acid. Based on the above three microbe samples' water contents and complex refractive indexes in far infrared band, biologic particle's complex refractive index model in 6—14 μm band are constructed and the model's reliability is analyzed. The construction of biologic particle's complex refractive index model has important significance in the development of rapid analysis and identification method of biologic samples, and the detection and identification technologies of biologic aerosol.
Keywords： optical parameters complex refractive index of microbe far infrared band