拉曼光谱和DSC研究人参皂苷分子与DMPC双层膜的作用

探讨药物与细胞膜的作用对于改善药物的药理活性、揭示药物的作用机制具有重要意义。利用差示扫描量热(DSC)和拉曼光谱技术对五种人参皂苷分子与DMPC磷脂双层膜的作用进行了实验研究。结果显示，在人参皂苷分子的作用下，DMPC的极性头部骨架构象没有发生变化。Rb1和Rh2增多了脂肪酰链中无序性的构象，增强了侧链排列的无序性，而三醇组皂苷Re，Rf和Rg1对DMPC双层膜的影响较小。DSC结果进一步表明，Rb1和Rh2均与DMPC双层膜发生了强相互作用，两皂苷分子使DMPC的相变温度显著降低，双层膜的流动性增强。Rf对DMPC双层膜的扰动作用要强于Re和Rg1。

Raman and DSC Spectroscopic Studies on the Interaction between Ginsenosides and DMPC Bilayer Membranes

Study the effect of drugs and biological membranes is of prime importance on understanding drugs’ curative effects and improving their biological properties. In this article, Raman spectrum has been combined with differential thermal scanning technology to discuss the relationship between five categories of ginsenoside molecules and DMPC bilayer films. Raman results indicated that the saponin molecules have not altered the polarity conformation of O—C—C—N+ backbone in DMPC bilayers, and the polarity head still paralleled to the membrane surface. The order of the internal molecular chain and the lateral chain-chain packing have been decreased as the panaxadiol saponins Rb1 and Rh2 increased, and to the opposite, the panaxatriol saponins Re, Rf and Rg1have showed weak effects on DMPC bilayers. The DSC showed further results that the strong effects of ginsenoside Rb1 and Rh2 on DMPC, which both have obviously reduced the DMPC molecular phase transition temperature, thus increasing the fluidity of bilayers. In addition, panaxatriol saponin Rf has displayed stronger disturbance effect on DMPC than Re and Rg1.