成年雄性斑胸草雀脑多巴胺D1受体的分布

多巴胺是脑内关键的神经递质,它通过与多巴胺受体的作用及其下游的一系列反应来影响基因表达、神经调节和行为活动.在成年鸣禽中,中脑多巴胺能神经元投射到X区、HVC和RA等鸣唱相关核团,释放多巴胺的量受一定社会情境的影响,从而表现出directed song和undirected song等不同鸣唱行为.获得斑胸草雀脑中多巴胺受体的表达情况,为与社会情境有关的鸣唱行为及其他和多巴胺相关的行为活动的神经机制探究提供了基础,并可促进行为学、电生理等方面的研究.我们发现D1受体在斑胸草雀脑中的分布与其mRNA的分布基本一致:在脑的绝大部分区域都有分布;主要鸣唱核团HVC和RA有表达,与其周围区域差异不明显;LMAN中表达量较少;DLM中的表达量较高,并与其周围区域差异明显.但是纹状体内的表达与其周围区域的差异性没有mRNA明显;GCT中的表达量较多,与周围区域差异明显.

Distribution of dopamine D1 receptor in the brain of adult male zebra finches

Dopamine is a key neurotransmitter in the brain ,it influences the gene expression ,neural regulation and activities ,through a series of reaction of dopamine receptors .In adult songbirds ,dopa-mine neurons in the midbrain project to song nuclei as X area ,HVC ,RA ,the amount of released do-pamine is influenced by the social context-dependent behaviors ,directed song and undirected song .In order to know the distribution of dopamine receptors in the zebra finch brain ,we use immunohisto-chemistry method .We found that the distribution of D1 receptor in the zebra finch brain is consistent with the distribution of mRNA .D1 receptor distributed in most areas of the brain .The vocal nuclei X area ,HVC and RA have expressed .The expression of LMAN is lower ;the expression of DLM is higher .However ,the difference expression of mRNA in the striatum and the surrounding region is not obvious ;more expressed in GCT ,and the difference with surrounding region is obvious .