Intramolecular Folding of PolyT Oligonucleotides Induced by Cooperative Binding of Silver(I) Ions |
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Authors: | Jinghua Hao Dong Cao Qiang Zhao Dapeng Zhang Hailin Wang |
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Affiliation: | 1.School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China;2.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;3.University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract: | Ag+-bridged T-Ag+-T was recently discovered in a Ag+-DNA nanowire crystal, but it was reported that Ag+ had little to no affinity to T nucleobases and T-rich oligonucleotides in solution. Therefore, the binding mode for the formation of this type of novel metallo base pair in solution is elusive. Herein, we demonstrate that Ag+ can interact with polyT oligonucleotides once the concentration of Ag+ in solution exceeds a threshold value. The threshold value is independent of the concentration of the polyT oligonucleotide but is inversely proportional to the length of the polyT oligonucleotide. The polyT oligonucleotides are intramolecularly folded due to their positively cooperative formation and the stack of T-Ag+-T base pairs, resulting in the 5′- and 3′-ends being in close proximity to each other. The intramolecular Ag+-folded polyT oligonucleotide has a higher thermal stability than the duplex and can be reversibly modulated by cysteine. |
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Keywords: | cooperative binding DNA intramolecular folding metal-mediated base pairs silver ions |
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