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用磷酸二异辛酯/正辛醇反胶束从面包酵母粗提液中萃取谷胱甘肽
引用本文:周小华,王东,郑声申.用磷酸二异辛酯/正辛醇反胶束从面包酵母粗提液中萃取谷胱甘肽[J].应用化学,2009,26(9):1088-1089.
作者姓名:周小华  王东  郑声申
作者单位:(1.重庆大学化学化工学院 重庆 400030;2.重庆市固体废物管理中心 重庆)
摘    要:采用磷酸二异辛酯(P204)/正辛醇反胶束液从面包酵母粗提液中萃取还原型谷胱甘肽(GSH),研究了各种因素对萃取率的影响以及水相pH值和P204浓度与GSH萃取率关系的数学模型。正交实验确定,GSH的最佳萃取条件是水相pH值2.0,K+浓度0.1 mol•L-1,萃取时间15 min,P204浓度0.16 mol•L-1,GSH单次的萃取率为33.1%,2次萃取率达到49.7%。

关 键 词:面包酵母  谷胱甘肽  反胶束萃取  P204/正辛醇反胶束  
收稿时间:2008-09-10
修稿时间:2009-02-16

Extraction of glutathione with reversed micelles
ZHOU Xiao-Hua,WANG Dong,ZHENG Sheng-Shen.Extraction of glutathione with reversed micelles[J].Chinese Journal of Applied Chemistry,2009,26(9):1088-1089.
Authors:ZHOU Xiao-Hua  WANG Dong  ZHENG Sheng-Shen
Institution:(1.College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400030; 2.Chongqing Solid Waste Management Center,Chongqing)
Abstract:he extraction of glutathione (GSH) from distillate of bread yeast with reversed micelles made up of phosphoric acid dioctyl ester (P204) and n-octanol was researched. The effect factors on Extraction rate of GSH were examined. The optimum conditions were confirmed in the orthogonal tests that extraction time is 15 min; pH value in aqueous phase is 2.0; concentration of P204 is 0.16 mol•L-1 and concentration of K+ is 0.1 mol•L-1. The extraction rate of GSH is 33.1% and the twi-extraction rate got 49.7% in the optimum conditions.
Keywords:Bread yeast  Glutathione (GSH)  Extraction with reversed micelles  reversed micelles of P204 / octanol
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