汉坦病毒感染宿主细胞后TRAIL受体的动态变化

两株汉坦病毒(HV 76/118株和H-114株)分别感染人胚肺成纤维细胞(HEPF)后,于感染后不同时间(6,24,96h)提取细胞总RNA,半定量RT-PCR的方法检测肿瘤坏死因子相关诱导凋亡配体(TRAIL)的相关受体(TRAIL-R1～TRAIL-R4)在汉坦病毒感染细胞过程中的表达变化,以明确TRAIL受体在汉坦病毒感染细胞过程中的表达情况.结果显示,正常细胞内TRAIL-R4表达最高,其次为TRAIL-R2.汉坦病毒(HV 76/118株和H-114株)感染HEPF 6h时仅TRAIL-R1表达显著上升,TRAIL-R2、TRAIL-R4表达先下降后上升并在96h达到高峰.HV 76/118株感染HEPF细胞6h时TRAIL-R3表达减低并维持在低表达水平,而HV H-114感染后仅24h出现短暂下降.结果表明,TRAIL受体表达在汉坦病毒感染过程中呈动态变化,且不同毒株的变化趋势存在差异.

Dynamic Expression of TRAIL Receptor in Hantavirus-Infected HEPF Cells

To investigate the expression pattern of TRAIL(TNF-related apoptosis-inducing ligand) receptor induced by hantavirus(HV) infection,RT-PCR was employed to half-quantitate the expression of the TRAIL receptors(TRAIL-R1-TRAIL-R4) in human embryonic pulmonary fibroblast(HEPF) which was infected with HV76-118 strain and H-114 strain at 6 h,24 h and 96 h,respectively.The results showed that normal HEPF cells exhibited the highest expression of TRAIL-R4,followed by that of TRAIL-R2.Only TRAIL-R1 was upregulated at 6 h post infection.The expression of TRAIL-R2 and R4 decreased at both 6 h and 24 h post infections.Both of them increased at 96 h subsequently.The expression of TRAIL-R3 was down-regulated in the HV76-118 infected group,but those in the HV H-114 infected group remained unchanged.In conclusion,the expression of TRAIL receptors show the dynamic change during the process of HEPF infected with HV,and a various pattern is exhibited in different subtype HV infected groups.