Amperometric determination of bovine insulin based on synergic action of carbon nanotubes and cobalt hexacyanoferrate nanoparticles stabilized by EDTA |
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Authors: | Fengli Qu Minghui Yang Yashuang Lu Guoli Shen Ruqin Yu |
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Institution: | (1) State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People’s Republic of China |
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Abstract: | A simple approach is proposed for the synthesis of cobalt hexacyanoferrate nanoparticles (CoNPs) with uniform shape and size
controlled by ethylene diamine tetraacetic acid (EDTA) as a stabilizer. A sensitive amperometric biosensor for insulin has
been prepared using glassy carbon electrodes by solubilization of carbon nanotubes (CNTs) in chitosan (CHIT) together with
CoNPs synthesized by the new methodology. The CoNP-CNT-CHIT organic–inorganic system exerts a synergistic effect, resulting
in the remarkably enhanced insulin currents owing to the superior electron-transfer ability of CNTs and the excellent reversible
redox centers of CoNPs. High-resolution transmission electron microscopy (HRTEM) was used to provide closer inspection of
the CoNPs. The effects of alkali metal cations and the concentrations of CNTs and CoNPs on the voltammetric behavior of the
film-modified electrode were also investigated. In pH 6.98 phosphate buffer (PB) at +0.7 V (vs. SCE) the insulin biosensor
exhibits a linear response range of 0.1–3 μM with a correlation coefficient of 0.98, and the detection limit (S/N=3) is determined
to be 40 nM, the stability of the biosensor was tested and found satisfactory. There is great promise for in vivo measurements
of this important hormone. |
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Keywords: | Cobalt hexacyanoferrate Carbon nanotubes Insulin Chitosan |
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