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Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction |
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| Authors: | Hiromi Horiuchi Noriya Iwami Akashi Ohtaki Tamotsu Zako Masafumi Yohda Toshiro Tani |
| Institution: | a Department of Applied Physics, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Kogane-i, Tokyo 184-8588, Japan b Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Kogane-i, Tokyo 184-8588, Japan c Strategic Research Initiative for Future Nano-Science and Technology, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Kogane-i, Tokyo 184-8588, Japan d Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1, Hirosawa, Wako, Saitama 351-0198, Japan |
| Abstract: | Feasibilities to stabilize CdSe/ZnS/trioctylphosphineoxide (TOPO) nanocrystals (quantum dots, QDs) in aqueous solutions with prefoldin macromolecules in their bioactive states are reported. Prefoldin is a jellyfish-shaped hexameric co-chaperone of the group II chaperonins. As a protein folding intermediate is captured within its central cavity, so CdSe/ZnS/TOPO QDs would also be included within this cavity. It is also found the QDs can be much more dispersed in aqueous solutions and suspended for certain period of time by adding trace amount of t-butanol in the buffer prior to the mixing of the QDs mother solution. While biochemical procedures are evaluated with ordinary fluorescence measurements, possible complex formations are also evaluated with TIRFM single-molecule detection techniques. |
| Keywords: | 81 07 Ta 82 39 Rt 87 14 Ee 87 57Nk |
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