Evaluation of procedures for solid-phase extraction of [125I]-methodone from serum on to discs and cartridges |
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Authors: | S Rudaz W Haerdi J -L Veuthey |
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Institution: | (1) Laboratoire de chimie analytique pharmaceutique, Pavillon des Isotopes, 20 Bd d'Yvoy, 1211, Genève 4, Switzerland;(2) Department de chimie minérale, analytique et appliquée, Science II, 30 Quai Ansermet, 1211, Genève 4, Switzerland |
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Abstract: | Summary Biological samples must be purified before chromatography to eliminate interfering compounds. Discs have recently been developed
to enhance the performance of solid-phase extraction (SPE) and reduce solvent consumption. This type of support is available
in all the bonded-phases typically found in conventional packed SPE devices. The process for solid-phase extraction of 125I]-methadone from water and serum (i.e. retention and losses of 125I]-methadone during sample loading, washing, elution, etc.) has been studied to determine the behaviour of this compound on
several extracting supports. For serum solutions we observed significant matrix effects on discs because of their low capacity.
This resulted in loss of 125I]-methadone during the loading step because the contact time between the analyte and the functional groups of the support
was too short. Although mixed-mode discs, which have both hydrophobic and cation-exchange extraction mechanisms, do not perform
as well as conventional cartridges, they can, nevertheless, be used before liquid chromatographic analysis because of their
several mechanical and economical advantages in comparison with cartridges. |
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Keywords: | Solid-phase extraction Extraction disc Extraction cartridge Mixed-mode bonded phase Radiolabelled [125I]-methadone |
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