Blood compatible heteratom-doped carbon dots for bio-imaging of human umbilical vein endothelial cells |
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| Authors: | Jian Zhong Xinmian Chen Miaoran Zhang Chaoxin Xiao Lulu Cai Waleed Ali Khan Kaixuan Yu Jiayi Cui Lin He |
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| Affiliation: | 1. Personalized Drug Therapy Key Laboratory of Sichuan Province, Department of Pharmacy, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China;2. State Key Laboratory of Heavy Oil Processing, Beijing Key Laboratory of Biogas Upgrading Utilization, College of New Energy and Materials, China University of Petroleum (Beijing), Beijing 102249, China;3. State Key Laboratory of Biotherapy and Cancer, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu 610041, China;1. Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, PR China;2. College of Material Science and Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China;3. Guangdong Provincial Engineering Technology Research Center For Transparent Conductive Materials, South China Normal University, Guangzhou, 510006, China;1. Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006,China;2. Department of Chemistry, Washington State University, Pullman, WA 99164, USA |
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| Abstract: | Carbon dots have unique advantages in biological applications owing to their excellent optical properties. However, the biosafety evaluation of carbon dots has limitations owing to cytotoxicity in vitro, and there is little pre-safety evaluation before in vivo and clinical applications. Whether the carbon dots are or not suitable for applications in vivo, evaluation analysis can be made based on hemolysis and changes in erythrocyte morphology. In this work, a green fluorescent N, S-doped carbon dots (N, S-CDs) were obtained by hydrothermal method, tobias acid, and m-phenylenediamine as precursors. N, S-CDs not only possessed excellent dispersibility, uniform particle size, high quantum yield (37.2%) and stable photoluminescence property but also retain their photostability and strong fluorescence intensity in the acid/alkaline solutions, different ionic strengths (NaCl) and under 365 nm UV illumination. Moreover, the N, S-CDs displayed low cytotoxicity and high cellular uptake efficiency in human umbilical vein endothelial cells (HUVEC) and excellent blood compatibility to the erythrocyte. It is foreseeable that N, S-CDs could be further studied as a promising biological imaging agent in vivo. |
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| Keywords: | Carbon dots Fluorescent Cell imaging Erythrocyte Blood compatibility |
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