Conversion of aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase, a metalloenzyme, into a nonmetalloenzyme |
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| Authors: | Li Jingjing Wu Jing Fleischhacker Angela S Woodard Ronald W |
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| Affiliation: | Department of Medicinal Chemistry and Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065, USA. |
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| Abstract: | The Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS), a class II metalloenzyme, is converted into an active nonmetalloenzyme by a single amino acid mutation, namely, C11N. The result may provide insight into the evolutionary link between the two KDO8PS classes as well as the potential role of the metal and/or asparagine in the catalytic mechanism. |
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