A mechanism for the loss of 60 u from peptides containing an arginine residue at the C-terminus |
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Authors: | Michael J Deery Scott G Summerfield Armelle Buzy Keith R Jennings |
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Institution: | 1. Institute of Mass Spectrometry, Department of Chemistry, University of Warwick, Coventry, UK
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Abstract: | The loss of 60 u from protonated peptide ions containing an arginine residue at the C-terminus has been investigated by means of low energy tandem mass spectrometry. The lowest energy conformation of singly charged bradykinin is thought to involve a salt-bridge structure, which may lead to the formation of two isomeric forms. It is thought that one isomer retains the ionizing proton at the C-terminal end of the peptide, leading to the formation of the b n?1 + H + OH]+ fragment ion, and the other isomer retains the charge at the N-terminus, leading to the formation of the M + H ? 60]+ fragment ion. It was found that the formation of the M + H ? 60]+ ion occurs only from singly charged precursor ions. In addition, the loss of 60 u occurs from peptides in which the charge is localized at the N-terminus. These results indicate that the mechanism of formation of the M + H ? 60]+ ion may be driven by a charge-remote process. |
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