| 溶液pH和阳离子对端粒G-四链体DNA形成和结构的影响 |
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| 引用本文： | 周江,袁谷.溶液pH和阳离子对端粒G-四链体DNA形成和结构的影响[J].化学学报,2007,65(16):1728-1732. |
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| 作者姓名： | 周江 袁谷 |
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| 作者单位： | (北京大学化学与分子工程学院化学生物学系 北京分子科学国家实验室 生物有机与分子工程教育部重点实验室 北京 100871) |
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| 摘 要： | 采用电喷雾质谱和圆二色谱研究了溶液pH和阳离子对人类端粒G-四链体DNA的影响. ESI-MS和CD谱图表明, pH可以引起G-四链体DNA的构象转变和离解, 而K＋, NH4＋,阳离子对G-四链体DNA的形成有着重要的促进作用.
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| 关 键 词： | 端粒 G-四链体DNA ESI-质谱法 圆二色谱 |
| 收稿时间： | 2007-3-29 |
| 修稿时间： | 2007-03-292007-06-21 |
Effect of pH and Cations on the Formation and Structure of Human Telomeric G-Quadruplex DNA |
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| ZHOU,Jiang,YUAN,Gu.Effect of pH and Cations on the Formation and Structure of Human Telomeric G-Quadruplex DNA[J].Acta Chimica Sinica,2007,65(16):1728-1732. |
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| Authors: | ZHOU Jiang YUAN Gu |
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| Institution: | (Beijing National Laboratory for Molecular Sciences and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871) |
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| Abstract: | Electrospray ionization mass spectrometry and circular dichroism were used to investigate the factors which affect the motif of the human telomeric quadruplex,such as pH and cations.ESI-MS and CD spectra indicated that pH could induce both the conformational transition and the dissociation of the quadru-plex.Moreover,K and NH 4 cations play an important role in the formation of the quadruplex. |
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| Keywords: | telomere G-quadruplex DNA ESI mass spectrometry circular dichroism |
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