Organometallic activation of a fluorogen for templated nucleic acid detection |
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| Authors: | Franzini Raphael M Kool Eric T |
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| Institution: | Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA. |
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| Abstract: | A nucleic acid detection scheme that employs DNA-mediated delivery of an organomercury activator to unmask a fluorophore is described. The approach relies on adjacent hybridization of two oligonucleotide conjugates containing organomercury and caged rhodamine functionalities. Postsynthetic conjugation of amino-modified DNAs enabled efficient preparation of these probes. Complementary DNA templates yielded fluorescence signals arising from metal-assisted rhodamine uncaging. |
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