| 核黄素与NADH在紫光波段的激光诱导荧光光谱研究 |
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| 引用本文： | 葛琳琳,丁蕾,闫静,王颖萍,郑海洋,方黎.核黄素与NADH在紫光波段的激光诱导荧光光谱研究[J].原子与分子物理学报,2012,29(6). |
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| 作者姓名： | 葛琳琳 丁蕾 闫静 王颖萍 郑海洋 方黎 |
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| 作者单位： | 中国科学院安徽光机所,中国科学院安徽光机所,中国科学院安徽光机所,中国科学院安徽光机所,中国科学院安徽光机所,中国科学院安徽光机所 |
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| 基金项目： | 中国科学院合肥物质科学研究院院长基金 |
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| 摘 要： | 基于细胞代谢荧光体的激光诱导荧光探测,在生物反应过程监测与生物活性物质探测中具有广泛的应用.本文利用波长可调谐激光光源,结合液体射流进样装置,研究了核黄素与NADH等生物荧光体在紫光波段(389nm～404nm)激发的荧光光谱,并考察了激光强度、样品浓度等参数对荧光光谱特性的影响.实验观察到核黄素的激发光谱在402.5nm处出现“波谷”,具有特征性,选择403.5nm激光激发,核黄素的浓度灵敏度约为NADH的八百分之一.这些结果为发展生物荧光探测与识别技术提供了新的基础数据.
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| 关 键 词： | 激光诱导荧光 核黄素 NADH 液体射流 |
Study Laser Induced Fluorescence of Riboflavin and NADH |
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| Abstract: | Laser-induced fluorescence measurement based on cell metabolism fluorescent has been applied widely in biological reaction process monitoring and detection of biological active substances. In this paper, we use wavelength tunable laser light source combined with liquid jet injection device to study the fluorescence spectroscopy of Riboflavin and NADH excited in purple band (389nm ~ 404nm) , and study the influence of the laser intensity , sample concentration to the fluorescence characteristics. We observe a characteristic "valley" at 402.5nm of excitation spectra of Riboflavin. Choosing 403.5nm excitation, riboflavin concentration detection limit is about one eight-hundredth of NADH. These results provide new data base for the development of bioluminescence detection and identification technology. |
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| Keywords: | LIF Riboflavin NADH Liquid jet |
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