High-performance liquid chromatographic separation of the fat-soluble vitamins in cod liver oil and feeds |
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| Authors: | RR Elton-Bott CI Stacey |
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| Institution: | Department of Chemistry, Western Australian Institute of Technology, South Bentley, Western Australia 6102 Australia |
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| Abstract: | A MicroPak-CN column with a methylene chloride—chloroforms-n-hexane (3:2:15) mobile phase separates the fat-soluble vitamins in cod liver oil and formulated feed. Powdered feed samples are dissolved in n-hexane—chloroform—ethanol (6:3.5:0.5) and centrifuged before injection. The oil is dissolved in the extraction solvent or mobile phase before injection. The naturally occurring vitamins are identified from their absorption spectra by stopped-flow spanning of the individual vitamins. |
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