A fluorimetric determination of lecithin-cholesterol acyltransferase in blood plasma |
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| Authors: | Masatake Iwasaki Chizuko Hamada Kiyoshi Zaitsu Yosuke Ohkura |
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| Affiliation: | Daüchi College of Pharmaceutical Sciences, Tamagawa, Minami-ku, Fukuoka 815 Japan;Faculty of Pharmaceutical Sciences, Kyushu University, 62 Maidashi, Higashi-ku, Fukuoka 812 Japan |
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| Abstract: | A fluorimetric assay for lecithin-cholesterol acyltransferase in human plasma (normal and pathological) is described. The lowered cholesterol concentration in the enzyme reaction is determined fluorimetrically by using the cholesterol oxidase—peroxidase —tyramine system. The assay requires only 10 μl of plasma. |
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