Studying the toxic effect of cadmium and hexavalent chromium on microbial activity of a soil and pure microbe |
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Authors: | J Yao F Wang L Tian Y Zhou H L Chen K Chen N Gai R S Zhuang T Maskow B Ceccanti G Zaray |
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Institution: | 1. Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education & Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, 430074, Wuhan, P. R. China 2. UFZ Centre for Environmental Research Leipzig, 04318, Leipzig, Germany 3. Institute of Ecosystem Studies, Italian National Research Council (ISE-CNR), Area diella Ricerca, Via G. Moruzzi 1, 56124, Pisa, Italy 4. Department of Chemical Technology and Environmental Chemistry, E?tv?s University, 1518, Budapest, P.O. Box 32, Hungary
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Abstract: | Using TAM III multi-channel calorimetry combined with direct microorganism counting (bacteria, actinomycetes and fungi) under
laboratory conditions, we determined the microbial population count, resistance and activity toward cadmium (Cd(II)) and hexavalent
chromium (Cr(VI)) toxicity in soil. The thermokinetic parameters, which can represent soil microbial activity, were calculated
from power-time curves of soil microbial activity obtained by microcalorimetric measurement. Simultaneous application of the
two methods showed that growth rate constant (k), peak-heat output power (P
max) and the number of living microorganisms decreased with increasing concentration of Cd and Cr. The accumulation of Cr on
E. coli was conducted by HPLC-ICP-MS. Cr6+ accumulation by Escherichia coli was increased steadily with increasing Cr6+ concentration. The results revealed that the change in some thermo-kinetic parameters could have good corresponding relationship
with metal accumulation. Our work also suggests that microcalorimetry is a fast, simple, more sensitive, on-line and in vitro
method that can be easily performed to study the toxicity of different species of heavy metals on microorganism compared to
other biological methods, and can combine with other analytic methods to study the interaction mechanism between environmental
toxicants and microbes. |
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Keywords: | cadmium Escherichia coli hexavalent chromium HPLC-ICP-MS microcalorimetry soil microbial activity |
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