Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography |
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Authors: | H. Inoue Y. Nonomura A. Ashizawa I. Tajima M. Nomura N. Nakamura N. Yoshioka |
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Affiliation: | (1) Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, 223 Yokohama, Japan |
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Abstract: | Summary Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25 75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a. |
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Keywords: | Column liquid chromatography Bacteriochlorophylla Copper(II) bacteriochlorophylla Zinc(II) bacteriochlorophylla |
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