干灰化结合偏硼酸锂碱熔灰分ICP-AES测定生物样品中的硅铝等元素

探讨不同种类生物样品中Si和Al等10种元素的测定方法,采用干灰化结合偏硼酸锂碱熔灰分的前处理方法,用电感耦合等离子体原子发射光谱法(ICP-AES)同时测定试样中Si和Al以及Ca,Mg,Fe,Na,P,Mn,Sr,Ti。方法检出限Si为0.15μg·mL-1,Al为0.03μg·mL-1。用国家一级标准物质GBW07604(杨树叶),验证了方法的准确度及精密度,Si和Al的测定的准确度(RE)分别为-0.03%和-3%,Si和Al测定的方法精密度均小于3%,该方法已用于新研制的系列生物标准物质的定值,并取得满意结果。

Determination of Si, Al and Other in Biological Samples by ICP-AES with Fusion with LiBO2 after Dry Ashing

An alkaline sample digestion procedure using fusion with lithium metaborate (LiBO2) after dry ashing was developed. Silicon, aluminium and other, a total of 10 elements in different kinds of biological samples, were measured by ICP-AES. The detection limit of this method was 0.15 microg x mL(-1) for Si and 0.03 microg x mL(-1) for Al. The accuracy and precision of the method were demonstrated by analyzing certified reference materials poplar leaves (GBW07604). The precision was less than 3% and the accuracy was -0.03% for Si and -3% for Al. This method was applied to the certification of a series of ten biological reference materials. The results were satisfied.