A thermodynamic study on the binding of mercury and silver ions to urease |
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Authors: | Email author" target="_blank">G?Rezaei BehbehaniEmail author A?A?Saboury A?Taherkhani L?Barzegar A?Mollaagazade |
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Institution: | (1) Chemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran;(2) Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;(3) Member of Young Researchers Club, Islamic Azad University, Takestan Branch, Takestan, Iran |
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Abstract: | In this article, a thermodynamic study on the interaction of Jack bean urease, JBU, with
\textHg 2+ {\text{Hg}}^{ 2+ } and
\textAg + {\text{Ag}}^{ + } ions were studied by isothermal titration calorimetry (ITC) at 300 and 310 K in 30 mM Tris buffer solution, pH 7.0. The heats
of
\textJBU + \textHg 2+ {\text{JBU}} + {\text{Hg}}^{ 2+ } and
\textJBU + \textAg + {\text{JBU}} + {\text{Ag}}^{ + } interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are a set of
12 identical and non-cooperative sites for
\textHg 2+ {\text{Hg}}^{ 2+ } and
\textAg + {\text{Ag}}^{ + } ions. The binding of
\textHg 2+ {\text{Hg}}^{ 2+ } and
\textAg + {\text{Ag}}^{ + } ions with JBU are exothermic with association equilibrium constants of 5415.65 and 4368.15 for
\textAg + {\text{Ag}}^{ + } and 2389 and 2087 M - 1 M^{ - 1} for
\textHg 2+ {\text{Hg}}^{ 2+ } at 300 and 310 K, respectively. |
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Keywords: | |
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