Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids |
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| Authors: | Bahareh DabirmaneshKhosro Khajeh Jafar AkbariHanieh Falahati Somayeh DaneshjooAkbar Heydari |
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| Institution: | a Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iranb Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iranc Department of Biotechnology, Faculty of Sciences, Tehran University, Tehran, Irand Department of Microbiology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran |
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| Abstract: | The use of ionic liquids to replace organic solvents in biocatalytic processes has recently gained much attention. Despite the wide applications of oxidoreductases, there are few reports of their catalyzed reaction in ionic liquid. We have investigated the influence of four water miscible ionic liquids on the activity, stability and structure of the mesophilic alcohol dehydrogenase from yeast. Upon changes in ionic liquids concentration, both activity and stability of the enzyme were affected. As the concentration of ionic liquids increased, Km increased while kcat decreased. Associated conformational changes caused by ILs (150 mM) were monitored using fluorescence technique. Finally, the effects of ILs cations and anions on the enzyme activity and stability in aqueous IL mixtures were discussed. |
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| Keywords: | Activity Alcohol dehydrogenase Fluorescence Ionic liquids Stability |
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