Preparation of a polyglycol-C8 bonded phase and its characteristics |
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Authors: | H -X Zhang M -C Liu P -L Zhu |
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Institution: | (1) Department of Chemistry, Lanzhou University, 730000 Lanzhou, P. R. China |
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Abstract: | Summary A new bonded phase containing both non-polar C8 and polar polyglycol chains was synthesized in two steps. Silica was first silanized with octyltrimethoxysilane and then
the intermediate product was reacted with tetraethylene glycol to introduce the polar group. The bonded phase exhibits a selectivity
different from that of C8 or C18 reversed phases with a mixed-mode retention mechanism. The silanol effect on the polyglycol-C8 bonded phase was examined with the test mixture proposed by Engelhardt and co-workers. The experimental results indicate
that the new bonded phase displays less silanol effect. The observed tailing of some ionizable species resulted mainly from
their partial dissociation at the pH values of eluents close to thepK
a values of the solutes. Some potential applications of the bonded phase are also given. |
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Keywords: | Column liquid chromatography Bonded phases Mixed-mode separation Silanol effect |
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