Determination of nitroimidazole residues in porcine urine by liquid chromatography/tandem mass spectrometry |
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Authors: | Xia Xi Li Xiaowei Shen Jianzhong Zhang Suxia Ding Shuangyang Jiang Haiyang |
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Affiliation: | China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing, 100094, People's Republic of China. |
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Abstract: | A reliable and sensitive method was developed for determination of nitroimidazoles in porcine urine. Sample preparation involves an extraction with ethyl acetate, followed by a solid-phase extraction cleanup step. The final extract is injected into the liquid chromatography/tandem mass spectrometry system in the positive-ion electrospray ionization mode. A C8 column with water and acetonitrile as the mobile phase is used for chromatographic separation under gradient conditions. Overall average recoveries ranged from 83 to 107%. The limits of detection ranged from 0.03 to 0.05 ng/mL, and the limits of quantitation, from 0.1 to 0.2 ng/mL. |
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