| 苏云金芽孢杆菌SFZZ－03菌株发育过程的电镜观察 |
| |
| 引用本文： | 刘忱,郭志红.苏云金芽孢杆菌SFZZ－03菌株发育过程的电镜观察[J].电子显微学报,2016(5):436-439. |
| |
| 作者姓名： | 刘忱 郭志红 |
| |
| 作者单位： | 1. 中国医科大学细胞生物教研室,辽宁 沈阳,110122;2. 国家林业局森林病虫害防治总站,辽宁 沈阳,110034 |
| |
| 摘 要： | 本文应用透射电子显微镜观察了在林业方面具有开发利用价值的苏云金芽孢杆菌SFZZ03菌株在牛肉膏蛋白胨液体培养基中的发育历期。本实验在发酵的不同时间进行取样，经过电镜样品前期处理，在透射电镜下观察SFZZ03菌株的细胞分裂，芽孢、伴孢晶体的形成，不仅详细阐述了SFZZ03菌株的发育过程，而且初步判断了伴孢晶体、芽孢的最大产量期，为今后的大规模生产确定种子罐培养时间、发酵罐培养时间提供了参考数值。同时也为筛选高产量伴孢晶体菌株提供了一种有效的测定方法。
|
| 关 键 词： | 苏云金芽孢杆菌 透射电子显微镜 伴孢晶体 芽孢 发育过程 |
Observation on development process of Bacillus thuringiensis SFZZ-03 |
| |
| Abstract: | This research focuses on the development duration of Bacillus thuringiensis SFZZ?03 which has exploitation and utilization value in forestry in LB medium. In this experiment, samples were prepared at different times of fermentation, and then the development duration of Bacillus thuringiensis SFZZ?03 which contains cell division, sporulation and parasporal crystal formation were observed. This research not only provides details on development process of Bacillus thuringiensis SFZZ?03, but preliminarily analyzes the mass production of parasporal crystals and spores. It provides a reference value in determining tank culture time and fermenter time for large scale production in the furthure. It also provides an effective measuring method for screening high yield strains with parasporal crystals. |
| |
| Keywords: | Bacillus thuringiensis transmission electron microscope parasporal crystals sporulation development process |
| 本文献已被 万方数据 等数据库收录！ |
