甘草多糖螺旋结构的原子力显微镜研究

用原子力显微镜(AFM)对甘草多糖的微观结构进行观察, 实验结果表明, 甘草多糖主要由葡萄糖、阿拉伯糖和半乳糖组成. 甘草多糖分子的稀溶液铺展在Ni^2＋处理的云母片上, 经干燥, 乙醇固定后, 获得稳定、重复的图像. 甘草多糖分子具有高度分枝的结构, 并且糖链间形成环状、柱状或近似于螺旋状的结构. 甘草多糖链呈多股紧密的螺旋结构, 这种现象可能与该多糖中分子间的Van der Waals相互作用以及糖链间氢键缔合有关.

A Study of Helical Structure of Glycyrrhiza Polysaccharides by Atomic Force Microscope

A method has been developed to give topography of glycyrrhiza polysaccharides by atomic force microscopy (AFM). The results showed that glycyrrhiza polysaccharides mainly consist of glucose, arabi- nose, galactose, but the conclusions about the main chains are different. Aqueous solutions of the polysac- charides are deposited as drops onto Ni2 mica surfaces, air dried, and ethanol fixed, and then stable and re- producible images were captured. Glycyrrhiza polysaccharides have a multi-branched structure and a variety of different linkages between adjacent monosaccharides, which compose the small rings and the helical structure. From the images, glycyrrhiza polysaccharides form ordered multiple helical structure. The phe- nomenon may be related to van der Waals interaction and hydrogen bond of polysaccharide chains.