手性金属配合物d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层模型的转运吸收

采用动力学研究方法测定了手性金属配合物d-[Co(EDTA)]-和l-[Co(EDTA)]-跨Caco-2细胞单层的转运速率. 研究发现, 由于金属配合物的手性差异, 导致d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层的转运速率明显不同; 手性金属配合物经Caco-2细胞单层转运吸收时存在手性选择性, 表明小肠对手性金属配合物药物可能有选择性吸收; d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层的转运依赖浓度梯度驱动, 说明该对映体配合物经Caco-2细胞单层转运吸收时存在简单扩散的转运方式.

Transport and Absorption of Chiral Metal Complexes d-[Co(EDTA)] -and l-[Co(EDTA)] - Across Caco-2 Cell Monolayers

The trasport rate of chiral complexes d-[Co(EDTA)]-and l-[Co(EDTA)]-across Caco-2 cell monolayers were measured by the kineuics method. The studies discover that there is an obvious difference about the trasport rate of d-[Co(EDTA)]-and l-[Co(EDTA)]-when they get across Caco-2 cell monolayers because of the chiral difference of the metal complexes. The results show that there is a chiral selectivity when chiral metal complexes were transported and absorbed across Caco-2 cell monolayers and imply that intestine would selectively absorb drugs of chiral metal complexes. The trasport rate of d-[Co(EDTA)]-and l-[Co(EDTA)]-passing through Caco-2 cell monolayers depends on the grads of the complexes concentration, it is shown that their transport model is as simple diffuse when they were transported and absorbed through Caco-2 cell monolayers.