Resonance energy transfer methods of RNA detection |
| |
Authors: | Kyle A Cissell Eric A Hunt Sapna K Deo |
| |
Institution: | (1) Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, 402 N. Blackford St. Room LD 326, Indianapolis, IN 46202, USA |
| |
Abstract: | Quantitation of RNA is important in diagnostics, environmental science, and basic biomedical research. RNA is considered a
signature for pathogen identification, and its expression profile is linked with disease pathogenesis, allowing for biomarker
identification. RNA-based diagnostics is an emerging field of research. This expansion of interest in studying RNA has generated
demand for its accurate and sensitive detection. Several methods have therefore been developed to detect RNA. Resonance energy
transfer methods of RNA detection are highly promising in terms of simplicity and high sensitivity. In this review, we have
focused on the latest developments in resonance energy transfer methods of RNA detection that utilize various probe designs.
The probe designs discussed here are molecular beacons, quenched autoligation probes, and linear oligonucleotide probes. Resonance
energy transfer methods based on both fluorescence and bioluminescence detection are discussed. |
| |
Keywords: | RNA detection Molecular beacons Quenched autoligation probes Fluorescence resonance energy transfer Bioluminescence resonance energy transfer |
本文献已被 PubMed SpringerLink 等数据库收录! |
|