BIOCHEMICAL ISOLATION AND SPECTROSCOPIC CHARACTERIZATION OF POSSIBLE PHOTORECEPTOR PIGMENTS FOR PHOTOTAXIS IN A FRESHWATER Peridinium |
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| Authors: | Donat-P Häder Shi-Mei Liu |
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| Institution: | Institut für Botanik and Pharmazeutische Biologie der Friedrich-Alexander-Universität, Staudtstr. 5, D-8520 Erlangen, Germany |
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| Abstract: | Photoreceptor pigments have been isolated biochemically from the freshwater dinoflagellate Peridinium gatunense, and characterized spectroscopically. At least four different chromoproteins can be detected in the crude extract and the membrane fraction isolated from the cells absorbing at 580, 638, 667 and 710 nm, which correspond with the maxima in the action spectrum for phototaxis in this organism. Light energy absorbed by shorter wavelength pigments is emitted as fluorescence at wavelengths which are absorbed by pigments with maxima at longer wavelengths. Protein separation on a MonoQ anion exchanger column using fast liquid chromatography resulted in a non-bound fraction and four bound fractions eluted by an NaCI gradient, which differed in their pigment composition. |
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