| Studies on Fragmentation Pathways of N-Ethoxy （phenyl） phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry |
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| 作者姓名： | CAO Shu-xia LI Hong-wei GUO Yan-chun LIAO Xin-cheng ZHAO Yu-fen |
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| 作者单位： | CAO Shu-xia 1，LI Hong-wei 1，GUO Yan-chun 1，LIAO Xin-cheng 1 and ZHAO Yu-fen 1，2**1. The Key Laboratory of Chemical Biology and Organic Chemistry，Department of Chemistry，Zhengzhou University，Zhengzhou 450052，P. R. China;2. The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology，Ministry of Education，Department of Chemistry，School of Life Sciences and Engineering，Tsinghua University，Beijing 100084，P. R. China |
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| 摘 要： | IntroductionPhosphorylation often acts as a molecular switchcontrolling the protein activity in different pathways asin metabolism,signal transduction,cell division,andso on.Therefore,N-phosphoryl amino acids play aspecial and important role in biological…
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| 关 键 词： | N-乙氧基（苯基）磷酰基氨基酸 电子喷射电离离子阱质谱分析 串联质谱法 分裂 |
| 文章编号： | 1005-9040（2006）-05-598-04 |
| 收稿时间： | 2005-08-24 |
Studies on Fragmentation Pathways of N-Ethoxy (phenyl) phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry |
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| CAO Shu-xia LI Hong-wei GUO Yan-chun LIAO Xin-cheng ZHAO Yu-fen.Studies on Fragmentation Pathways of N-Ethoxy (phenyl) phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry[J].Chemical Research in Chinese University,2006,22(5):598-601. |
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| Authors: | Shu-xia CAO Hong-wei LI Yan-chun GUO Xin-cheng LIAO Yu-fen ZHAO |
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| Institution: | aThe Key Laboratory of Chemical Biology and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, P. R. China;bThe Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, P. R. China |
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| Abstract: | The positive and negative ESI-MS/MS spectra of N-ethoxy(phenyl) phosphoryl amino acids(EPP-AA) were investigated by electrospray ionization(ESI) ion trap mass spectrometry. The fragmentation pathways of M Na] and M-H] - ions are proposed and rationalized. The observation may have some potential applications in the interpretation of the MS/MS spectra of novel N-phosphoryl compounds. The complexity of MS/MS spectra of EPP-AA M Na] ions is decreased compared with that of N-dialkyloxyphosphoryl amino acid. Therefore, the new phos- phonamidate method may be considered one of the superior methods that can be used in sequencing peptides and proteins extensively. |
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| Keywords: | N-Ethoxy(phenyl)phosphoryl amino acid Electrospray ionization ion trap mass spectrometry Tandem mass spectrometry |
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