Analysis of phenolic constituents of biological interest in red wines by high-performance liquid chromatography |
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Authors: | López M Martínez F Del Valle C Orte C Miró M |
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Institution: | Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Spain. |
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Abstract: | We describe a reversed-phase HPLC method that uses gradient elution and diode array detection to determine four biologically active phenolic constituents of red wines: gallic acid, trans-resveratrol, quercetin and rutin. The method permits direct injection without sample pre-treatment. ODS Hypersil served as the stationary phase; the gradient was formed by acetic acid, methanol, and water. Each analysis required an equilibration period of 10 min and a run time of 50 min for completion. Previously, total phenols were analysed according to the Folin-Ciocalteu method, using gallic acid as the standard, and the results are given as gallic acid equivalent. |
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