Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect |
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| Authors: | Scherl Alexander Zimmermann-Ivol Catherine G Di Dio Joel Vaezzadeh Ali R Binz Pierre-Alain Amez-Droz Michel Cochard Roland Sanchez Jean-Charles Glückmann Matthias Hochstrasser Denis F |
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| Affiliation: | Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospitals, Geneva, Switzerland. alexander.scherl@medecine.unige.ch |
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| Abstract: | Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry. |
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