Location and base selectivity on fragmentation of brominated oligodeoxynucleotides |
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Authors: | Buré Corinne Castaing Bertrand Lange Catherine Delmas Agnès F |
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Institution: | Centre de Biophysique Moléculaire, CNRS UPR 4301, University of Orléans and INSERM, rue Charles Sadron, 45071 Orléans Cedex 02, France. bure@cnrs-orleans.fr |
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Abstract: | Bromine-modified oligodeoxynucleotides (ODNs) were fragmented in the electrospray source to study the influence of brominated bases on fragmentation. Several 13-mer ODNs containing a brominated pyrimidine base, BrdU (5-bromodeoxyuridine) or BrdC (5-bromodeoxycytidine), were analyzed. Low cone voltage fragmentation yields a loss of the brominated base with a preferential loss for the brominated base closer to the 5'-end (2-position > 4-position > 12-position) as well as a preferential loss of BrdU over BrdC. Higher cone voltage produces backbone fragmentation with complementary a(n)-base and w(m) ions close to the brominated base. On the basis of these observations, we located the brominated base in the sequence for all of the ODNs studied. |
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Keywords: | oligodeoxynucleotide fragmentation electrospray in‐source CID location selectivity base selectivity |
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