基于单壁碳纳米管-DNA酶复合结构的电化学生物传感器

结合DNA酶优异的氧化还原催化特性和碳纳米管的电化学特性, 制备了单壁碳纳米管-DNA酶复合材料, 并通过壳聚糖将其固定到玻碳电极表面构建了电化学生物传感界面. 研究了单壁碳纳米管-DNA酶复合结构的氧化还原反应催化特性, 并以此为传感平台构建了葡萄糖氧化酶电化学生物传感器. 结果表明, 单壁碳纳米管-DNA酶复合材料修饰的电极对过氧化氢的响应具有较宽的线性范围(5×10^-6~1×10^-2 mol/L)和良好的检测灵敏度(检出限为1×10^-6 mol/L). 采用制备的葡萄糖氧化酶传感器实现了对葡萄糖的快速灵敏检测.

Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors

Taking advantage of the excellent catalytic redox activity andthe electrochemical property of DNAzyme and carbon nanotubes, a novel nano-complex of single-walled carbon nanotubes(SWNTs)/DNAzyme was prepared, and the obtained nano-complex was finally immobilized on the surface of glass carbon electrode(GCE) with the assistance of chitosan to fabricate electrochemical biosensing interface. The catalytic property of the SWNTs/DNAzyme complex was explored, and a glucose oxidase biosensor was then prepared based on the fabricated electrochemical biosensing platform. The SWNTs/DNAzyme complex-based biosensing platform can direct response to hydrogen peroxide at low potential of -0.24 V with high sensitivity(the detection limit is 1?10^-6 mol/L) and wide linear range(5?10^-6-1?10^-2 mol/L), and the prepared glucose oxidase biosensor was used to fast and sensitive detection of glucose.