3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance |
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| Authors: | Sebastian Meier, Andrew J. Benie, Jens
. Duus,Ole W. S rensen |
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| Affiliation: | aCarlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark;bNovo Nordisk A/S, Diabetes Protein Engineering, DK-2760 Måløv, Denmark;cDepartment of Chemistry, Technical University of Denmark, Kemitorvet 207, DK-2800 Kgs. Lyngby, Denmark |
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| Abstract: | 3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons. |
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| Keywords: | 3D H2BC H2BC Multidimensional NMR Natural abundance Biomolecular NMR |
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