NO Fluorescence Quantification by Chitosan CdSe Quantum Dots Nanocomposites |
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| Authors: | Eliana F. C. Simões João M. M. Leitão Joaquim C. G. Esteves da Silva |
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| Affiliation: | 1. Faculdade de Farmácia da Universidade de Coimbra, Pólo das Ciências da Saúde, Centro de Investiga??o em Química da Universidade do Porto (CIQ-UP), 3000-548, Coimbra, Portugal
2. Departamento de Química, Faculdade de Ciências da Universidade do Porto, Centro de Investiga??o em Química da Universidade do Porto (CIQ-UP), R. Campo Alegre 687, 4169-007, Porto, Portugal
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| Abstract: | The quantification of nitric oxide (NO) based on the quenching of the fluorescence of a nanocomposites sensor constituted by cadmium/selenium quantum dots (CdSe) stabilized by chitosan (CS) and mercaptosuccinic acid (MSA) is assessed. The optimization of the response of the CS-CdSe-MSA nanocomposites to NO was done by multivariate response surface experimental design methodologies. The highest fluorescence quenching was obtained at pH 5.5 and at room temperature. The NO quantification capability of CS-CdSe-MSA was evaluated using standard solutions and a NO donor reagent. A large linear working range from 5 to 200 μM and a limit of detection of 1.86 μM were obtained. Better quantification results were obtained using the NO donor reagent. Besides NO, the response of the fluorescence of CS-CdSe-MSA to the main reactive oxygen and nitrogen species and similar NO compounds was also assessed. |
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