| 金属配位剂衍生-高效液相色谱法测定粮食及白酒中的游离氰化物 |
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| 引用本文： | 孟梁,申贵隽,张强.金属配位剂衍生-高效液相色谱法测定粮食及白酒中的游离氰化物[J].分析科学学报,2009,25(5). |
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| 作者姓名： | 孟梁 申贵隽 张强 |
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| 作者单位： | 1. 大连大学环境与化学工程学院,辽宁大连,116622
2. 中国刑事警察学院刑事科学技术系,辽宁沈阳,110035 |
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| 摘 要： | 采用金属配位剂Ni(Ⅱ)-NH3作为衍生剂,与游离氰化物反应生成稳定且具有高紫外吸收值的Ni(CN)_4~(2-),使用高效液相色谱-紫外检测法(HPLC-UV)检测游离氰化物.方法的线性范围为0.014～0.540 mg/L,相关系数R~2=0.9984,检出限为4.0 μg/L.该方法简便快速,抗干扰能力强,灵敏度高,适于食品、环境、生物等样品中游离氰化物的检测.
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| 关 键 词： | 游离氰化物 衍生化 高效液相色谱 粮食 白酒 |
Metal-ligand Reagent Derivatization and High-performance Liquid Chromatography Determination for Free Cyanide Analysis |
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| MENG Liang,SHEN Gui-jun,ZHANG Qiang.Metal-ligand Reagent Derivatization and High-performance Liquid Chromatography Determination for Free Cyanide Analysis[J].Journal of Analytical Science,2009,25(5). |
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| Authors: | MENG Liang SHEN Gui-jun ZHANG Qiang |
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| Institution: | MENG Liang1,SHEN Gui-jun1,ZHANG Qiang2(1.Environmental and Chemical Engineering College,Dalian University,Dalian 116622,2.Department of Forensic Science,China Criminal Police Unviersity,Shenyang 110035) |
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| Abstract: | A new method involving metal-ligand reagent derivatization and high-performance liquid chromatography (HPLC) was developed for the determination of free cyanide in grain and distilled spirit. The free cyanide reacted with Ni(Ⅱ)-NH_3 to form a stable and highly UV absorbing tetracyanonickelate anion, and then was determined by HPLC. The calibration curve was linear for CN~- in the range of 0.014～0.540 mg/L (R~2=0.9984). The detection limit (S/N=3) was estimated to be 4.0 μg/L for CN~-. This method is simple, rapid, low interference and with high sensitivity, which can be used for the determination of free cyanide in common food, environmental and physiological samples. |
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| Keywords: | Free cyanide Derivatization High-performance liquid chromatogrphy Grain Distilled spirit |
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