Properties and performance of glucoamylases for fuel ethanol production |
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Authors: | Bradley A Saville Chunbei Huang Vince Yacyshyn Andrew Desbarats |
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Institution: | (1) Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, Canada |
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Abstract: | Studies were conducted on maltodextrin saccharification and on simultaneous saccharification and fermentation (SSF) with various
commercial glucoamylases. In kinetics studies, none of the glucoamylases were able to completely convert maltodextrin into
glucose. Typically, about 85% conversion was obtained, and glucose yields were about 75%. Typically, the kinetics were biphasic,
with 1 h of rapid conversion, then a significant reduction in rate. Data were consistent with strong product inhibition and/or
enzyme inactivation. Some glucoamylases followed first-order kinetics, initially slower at dextrin conversion, but eventually
achieving comparable conversion and glucose concentrations. Most of the glucoamylases were more active at 55°C than at 35°C,
but pH had little effect on activity. Screening studies in an SSF system demonstrated little difference between the glucoamylases,
with a few exceptions. Subsequent targeted studies showed clear differences in performance, depending on the fermentation
temperature and yeast used, suggesting that these are key parameters that would guide the selection of a glucoamylase. |
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Keywords: | Glucoamylase kinetics fermentation saccharification |
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