Solid-state pH ultramicrosensor based on a tungstic oxide film fabricated on a tungsten nanoelectrode and its application to the study of endothelial cells |
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Authors: | Katsunobu Yamamoto Tianshu Zhou Min Zhu Takeshi Kato Litong Jin |
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Affiliation: | a Laboratory of Research and Development, Bioelectroanalytical System Inc., 1-36-6 Oshiage, Sumida-Ku, Tokyo, Japan b Department of Chemistry, East China Normal University, Shanghai 200062, China c Department of Environmental Science, East China Normal University, Shanghai 200062, China d Instrumental Analysis Center, Gifu University, Yanagido, Gifu 501-1193, Japan |
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Abstract: | In this paper, preparation of a novel pH ultramicrosensor and its physiological application has been discussed. A tungsten nanoelectrode was produced by an etching method in 0.1 mol/l NaOH solution at the potential of +0.4 V (versus Ag/AgCl reference electrode) for about 100 s and the diameters ranged from 500 to 800 nm. The pH ultramicrosensor was fabricated by producing WO3 at W nanoelectrode surface by electrooxidation in 2.0 mol/l H2SO4 solution between 1.0 and 2.0 V. At last, Nafion was coated on the surface of WO3 to protect the pH ultramicrosensor. The W/WO3 pH ultramicrosensor exhibited a good pH linear region from 2.0 to 12.0 with a super-Nernstian slope of −53.5 ± 0.5 mV/pH unit. Response times ranged from 3 s at about pH 6.0-7.0 up to 15 s at high pH. An interference of various ions to the pH measurement was also studied in this paper. We also studied the lifetime, stability and reproducibility of the W/WO3 pH ultramicrosensor. In order to testing the performance of W/WO3 ultramicrosensor, we applied it to measure the extracellular pH values and a pH variation was also given about the normal, damaged and recovery endothelial cells. |
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Keywords: | W/WO3 pH ultramicrosensor Endothelial cells |
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