Determination of 18 nucleobases, nucleosides and nucleotides in human peripheral blood mononuclear cells by isocratic solvent-generated ion-pair chromatography |
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| Authors: | Margit Cichna Heide Daxecker |
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| Affiliation: | a Institute of Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria
b Ludwig Boltzmann Institute for Cardiothoracic Research, Institute of Laboratory Diagnostics, Kaiser Franz Josef Hospital, Kundratstrasse 3, A-1100 Vienna, Austria |
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| Abstract: | The paper describes a method for the separation of 18 nucleotides, nucleosides and nucleobases by isocratic solvent-generated ion-pair chromatography in 55 min. It has been developed for pharmacodynamic monitoring of mycophenolic acid, the active metabolite of the immunosuppressant mycophenolate mofetil. The method was applied for the detection of mycophenolic acid-induced changes in inosine 5′-monophosphate dehydrogenase (IMPDH) activity in the lysate of human peripheral blood mononuclear cells. |
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| Keywords: | Solvent generation Ion-pair chromatography Inosine 5&prime -monophosphate dehydrogenase Mycophenolic acid Xanthosine 5&prime -monophosphate Peripheral blood mononuclear cells |
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