Ceramic-based multisite microelectrode array for rapid choline measures in brain tissue |
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| Authors: | Jason J Burmeister Greg A Gerhardt |
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| Institution: | a Departments of Anatomy, Neurobiology and Neurology, Center for Sensor Technology, Morris K. Udall Parkinson’s Disease Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536-0098, USA
b Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO, USA |
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| Abstract: | A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200 μM choline (R2=0.999±0.001) with a detection limit of approximately 0.4 μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4 s, allowing for rapid measures of choline. Good selectivity (>300:1) was observed over interferents such as ascorbic acid, uric acid, and DOPAC in the single microelectrode mode. However, a self-referencing recording mode was needed to remove potassium-evoked dopamine signals in rat striatum. In vivo measurements of choline in the rat brain are presented. |
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| Keywords: | Choline Neurotransmitters Voltammetry Biosensor CNS |
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